Patrice Hamel

Associate Professor
Faculty

KEYWORDS: Arabidopsis / Chlamydomonas / mitochondria / chloroplast / membrane biogenesis / respiration / photosynthesis / Complex I assembly / mitochondrial disease / disulfide bond formation / redox enzymes / hydrogen production

RESEARCH INTERESTS: Mitochondria and chloroplast biogenesis

Our laboratory is interested in the topic of membrane biogenesis, in particular membrane systems in chloroplasts and mitochondria that are specialized in the transduction of energy. We use the plant Arabidopsis thaliana and the freshwater alga Chlamydomonas reinhardtii as experimental systems.

One area of research aims to dissect the assembly of mitochondrial Complex I, an enzyme involved in electron transfer. The goal is to discover novel assembly factors for this multimeric enzyme whose dysfunction is the most prevalent cause of mitochondrial diseases in humans. The long-term objective is to elucidate the molecular basis of Complex I deficiencies in humans, most of which still remain unexplained to this day.

 

A second research focus is concerned with disulfide bond formation and reduction in the thylakoid lumen, a compartment critical for photosynthesis. Long considered to be an empty compartment, the thylakoid lumen has now gained considerable attention with the realization that numerous molecules reside there in addition to the previously known photosynthetic proteins. Our goals are to define the molecular identity of the redox enzymes promoting disulfide bond formation/reduction in the lumen and discover their relevant targets of action in vivo. More information about our research can be found by visiting our lab web site (see below for link).

 

 

Selected publications (2000 - Date):

  1. Corvest, V., Murray, D.A., Hirasawa, M., Knaff, D.B., Guiard, B. and Hamel, P. (2012) The flavoprotein Cyc2p, a mitochondrial cytochrome c assembly factor, is a NAD(P)H-dependent heme reductase. Mol. Microbiol. 83, 968-980.

  2. Karamoko, M., Cline.,S. Redding, K., Ruiz, N. and Hamel, P. (2011) Lumen Thiol Oxidoreductase 1, a disulfide bond forming catalyst, is required for the assembly of photosystem II in Arabidopsis. Plant Cell 23, 4462-4475.

  3. Barbieri, M.R., Larosa, V., Nouet, C., Subrahmanian, N., Remacle, C. Hamel, P. (2011) A Forward Genetic Screen Identifies Mutants Deficient for Mitochondrial Complex I Assembly in Chlamydomonas reinhardtiiGenetics 188, 349-358.

  4. Stephane T. Gabilly, Janette Kropat, Mohamed Karamoko, M. Dudley Page, Stacie S. Nakamoto, Sabeeha S. Merchant and Patrice P. Hamel (2011) A Novel Component of the Disulfide-Reducing Pathway Required for Cytochrome c Assembly in Plastids. Genetics Society of America. DOI: 10.1534/genetics.110.125369

  5. Stephane T. Gabilly, Beth Welty Dreyfuss, Mohamed Karamoko, Vincent Corvest, Janette Kropat, M. Dudley Page, Sabeeha S. Merchant, and Patrice P. Hamel (2010) CCS5, a Thioredoxin-like Protein Involved in the Assembly of Plastid c-Type Cytochromes*. JOURNAL OF BIOLOGICAL CHEMISTRYVOLUME 285•NUMBER 39•SEPTEMBER 24, 2010

  6. Noah M. Reynolds a,b, Jiqiang Ling b,c, Hervé Roy a,b, Rajat Banerjee a,b, Sarah E. Repasky a, Patrice Hamel c,d,e, and Michael Ibba a,b,c,1 (2010) Cell-specific differences in the requirements . (www.pnas.org/cgi/doi/10.1073/pnas.0909640107)

  7. Vincent Corvest,* Darren A. Murrey,* Delphine G. Bernard,†,1 David B. Knaff,‡ Bernard Guiard† and Patrice P. Hamel*,2 (2010) c -Type Cytochrome Assembly in Saccharomyces cerevisiae: A Key Residue for Apocytochrome c1/Lyase Interaction. Genetics Society of America DOI: 10.1534/genetics.110.120022. 

  8. Claire Remacle,1 Sara Cline,2 Layla Boutaffala,1† Stephane Gabilly,2 Ve´ronique Larosa,1,2 M. Rosario Barbieri,2 Nadine Coosemans,1 and Patrice P. Hamel2* (2009) The ARG9 Gene Encodes the Plastid-Resident N-Acetyl Ornithine Aminotransferase in the Green Alga Chlamydomonas reinhardtii. EUKARYOTIC CELL, Sept. 2009, p. 1460–1463.

  9. Bernard, D., Quevillon-Cheruel S., Merchant, S., Guiard, B. and Hamel, P. (2005) Cyc2p, a membrane-bound flavoprotein involved in the maturation of mitochondrial c-type cytochromes. J. Biol. Chem. 280, 39852-398549. [pdf]

  10. Page, M*., Hamel, P*., Gabilly, S., Zegzouti, H, Perea, J., Alonso, J., Ecker, J., Theg, S., Christensen, S., and Merchant, S. (2004) A homolog of the prokaryotic thiol-disulfide transporter CcdA is required for the assembly of the cytochrome b6f complex in Arabidopsis chloroplasts. J. Biol. Chem. 279, 32474-32482. (* equal contribution). [pdf]

  11. Hamel, P., Dreyfuss, B., Xie, Z., Gabilly, S. and Merchant S. (2003) Essential histidine and tryptophan residues in CcsA, a system II polytopic cytochrome c biogenesis protein. J. Biol. Chem. 278, 2593-2603. [pdf]Dreyfuss, B., Hamel, P., Nakamoto, S. and Merchant S. (2003) Functional analysis of a divergent system II protein, Ccs1, involved in c-type cytochrome biogenesis. J. Biol. Chem. 278, 2604-2613. [pdf]

  12. Bernard, D., Gabilly, S., Dujardin, G., Merchant, S. and Hamel, P. (2003) Overlapping specificities of the cytochrome c and c1 heme lyases. J. Biol. Chem. 50, 49732-49742. [pdf]Nakamoto, S., Hamel, P. & Merchant, S., (2000) Assembly of chloroplast cytochromes b and c. Biochimie


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Areas of Expertise
  • Mitochondria and Plastids
  • Biogenesis of Multimeric Enzymes
  • Thiol-sulfide Chemistry
Education
  • Ph.D., University of Orsay, Paris XI, France, 1999.

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Phone:
614-292-3817
Fax:
614-292-6345
582 Aronoff Laboratory
318 West 12th Avenue
Columbus, OH 43210