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Ohio State and Nationwide Children's Hospital Laboratories using the zebrafish model

Ohio State Zebrafish Links

Zebrafish Community Resources

Amacher Laboratory Protocols

  • CRISPR mutagenesis 3.0 - this is an older version of our CRISPR protocol
  • CRISPR mutagenesis 3.1 - this is the detailed protocol supplement to our paper: Talbot JC, Amacher SL (2014) "A streamlined CRISPR pipeline to reliably generate zebrafish frameshifting alleles." Zebrafish 11, 583-585.
  • CRISPR mutagenesis 3.2 - this is the published supplement with an additional page (p. 13) that provides another option for making gRNA using short oligos.
  • Blog forum for suggestions for and questions about our CRISPR protocol
  • More links coming soon!

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